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mtesr1 complete kit (basal medium plus 5× supplement)  (STEMCELL Technologies Inc)

 
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    Structured Review

    STEMCELL Technologies Inc mtesr1 complete kit (basal medium plus 5× supplement)
    Recommended number of cells per cryotube and number of wells to thaw a cryotube into for hPSCs, CPCs, EpiCs, and EpiC-FBs
    Mtesr1 Complete Kit (Basal Medium Plus 5× Supplement), supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mtesr%C2%AE+1+medium+kit%E2%80%94basal+medium+plus+5%C3%97+supplement/mtesr1+medium/pmc08991283-40-0-9
    Average 90 stars, based on 1 article reviews
    mtesr1 complete kit (basal medium plus 5× supplement) - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "Directed differentiation of human pluripotent stem cells to epicardial-derived fibroblasts"

    Article Title: Directed differentiation of human pluripotent stem cells to epicardial-derived fibroblasts

    Journal: STAR Protocols

    doi: 10.1016/j.xpro.2022.101275

    Recommended number of cells per cryotube and number of wells to thaw a cryotube into for hPSCs, CPCs, EpiCs, and EpiC-FBs
    Figure Legend Snippet: Recommended number of cells per cryotube and number of wells to thaw a cryotube into for hPSCs, CPCs, EpiCs, and EpiC-FBs

    Techniques Used: Cell Counting


    Figure Legend Snippet:

    Techniques Used: Immunofluorescence, Microscopy, Recombinant, Sterility, Modification, Knock-Out, Electron Microscopy, SYBR Green Assay, Cell Culture, Real-time Polymerase Chain Reaction, Cytometry, Filtration, Transferring

    mTeSR1 medium (commercially available mTSR1 complete kit)
    Figure Legend Snippet: mTeSR1 medium (commercially available mTSR1 complete kit)

    Techniques Used: Concentration Assay

    Related Articles

    Knock-Out:

    Article Title: Making and using in vitro-synthesized ssRNA for introducing into mammalian cells to induce a biological or biochemical effect
    Article Snippet: StainAlive DyLight 488 Mouse anti-Human Tra-1-60 Antibody (Stemgent, Cat#09-0068) Y27632 Rock I Inhibitor (Stemgent, Cat#04-0012) 10×PBS without calcium or magnesium (Lonza Biowhittaker, Cat#17-517Q, Thermo) Collagenase Type IV, 250 U/mg (GIBCO, Cat#17104-019) iMEF-irradiated mouse embryonic fibroblasts (R&D Systems Cat# PSC001) BD MATRIGELTM hESC-qualified Matrix (BD Cat#354277, Thermo) mTeSR® 1 Medium Kit—Basal Medium plus 5× Supplement (STEMCELL Technologies, Cat#05850) Dispase 5 mg/ml (STEMCELL Technologies, Cat#07913) Synth-a-Freeze, cell freezing media, (GIBCO, Cat# A12542-01, Life Technologies) DMEM/F12 (1:1) Media (GIBCO, Cat#11330, Life Technologies) KNOCKOUTTM SR Serum Replacement for ES cells (GIBCO, Cat#10828, Life Technologies) MEM Non-Essential Amino Acids Solution NEAA (100×) (GIBCO, Cat#11140, Life Technologies) Beta-mercaptoethanol (Sigma, Cat#63689) Alkaline Phosphatase Staining Kit II (Stemgent, Cat#00-0055) Bovine Serum Albumin (for FGFb) Paraformaldehyde 95% (Sigma, Cat#158127) Antibodies, wash buffers, etc iPS Cell Medium—DMEM/F12, 20% Knockout SR, 10 ng/ml FGFb, 1× non-essential amino acids, 1× Pen/Strep, 0.1 mM beta-Mercaptoethanol (bME), 1× GLUTAMAXTM mTeSR 1 Medium—mTeSR 1 plus 1× Supplement Materials and Methods for Characterizing iPSC Colonies Generated Using the Methods.

    Staining:

    Article Title: Making and using in vitro-synthesized ssRNA for introducing into mammalian cells to induce a biological or biochemical effect
    Article Snippet: StainAlive DyLight 488 Mouse anti-Human Tra-1-60 Antibody (Stemgent, Cat#09-0068) Y27632 Rock I Inhibitor (Stemgent, Cat#04-0012) 10×PBS without calcium or magnesium (Lonza Biowhittaker, Cat#17-517Q, Thermo) Collagenase Type IV, 250 U/mg (GIBCO, Cat#17104-019) iMEF-irradiated mouse embryonic fibroblasts (R&D Systems Cat# PSC001) BD MATRIGELTM hESC-qualified Matrix (BD Cat#354277, Thermo) mTeSR® 1 Medium Kit—Basal Medium plus 5× Supplement (STEMCELL Technologies, Cat#05850) Dispase 5 mg/ml (STEMCELL Technologies, Cat#07913) Synth-a-Freeze, cell freezing media, (GIBCO, Cat# A12542-01, Life Technologies) DMEM/F12 (1:1) Media (GIBCO, Cat#11330, Life Technologies) KNOCKOUTTM SR Serum Replacement for ES cells (GIBCO, Cat#10828, Life Technologies) MEM Non-Essential Amino Acids Solution NEAA (100×) (GIBCO, Cat#11140, Life Technologies) Beta-mercaptoethanol (Sigma, Cat#63689) Alkaline Phosphatase Staining Kit II (Stemgent, Cat#00-0055) Bovine Serum Albumin (for FGFb) Paraformaldehyde 95% (Sigma, Cat#158127) Antibodies, wash buffers, etc iPS Cell Medium—DMEM/F12, 20% Knockout SR, 10 ng/ml FGFb, 1× non-essential amino acids, 1× Pen/Strep, 0.1 mM beta-Mercaptoethanol (bME), 1× GLUTAMAXTM mTeSR 1 Medium—mTeSR 1 plus 1× Supplement Materials and Methods for Characterizing iPSC Colonies Generated Using the Methods.

    Generated:

    Article Title: Making and using in vitro-synthesized ssRNA for introducing into mammalian cells to induce a biological or biochemical effect
    Article Snippet: StainAlive DyLight 488 Mouse anti-Human Tra-1-60 Antibody (Stemgent, Cat#09-0068) Y27632 Rock I Inhibitor (Stemgent, Cat#04-0012) 10×PBS without calcium or magnesium (Lonza Biowhittaker, Cat#17-517Q, Thermo) Collagenase Type IV, 250 U/mg (GIBCO, Cat#17104-019) iMEF-irradiated mouse embryonic fibroblasts (R&D Systems Cat# PSC001) BD MATRIGELTM hESC-qualified Matrix (BD Cat#354277, Thermo) mTeSR® 1 Medium Kit—Basal Medium plus 5× Supplement (STEMCELL Technologies, Cat#05850) Dispase 5 mg/ml (STEMCELL Technologies, Cat#07913) Synth-a-Freeze, cell freezing media, (GIBCO, Cat# A12542-01, Life Technologies) DMEM/F12 (1:1) Media (GIBCO, Cat#11330, Life Technologies) KNOCKOUTTM SR Serum Replacement for ES cells (GIBCO, Cat#10828, Life Technologies) MEM Non-Essential Amino Acids Solution NEAA (100×) (GIBCO, Cat#11140, Life Technologies) Beta-mercaptoethanol (Sigma, Cat#63689) Alkaline Phosphatase Staining Kit II (Stemgent, Cat#00-0055) Bovine Serum Albumin (for FGFb) Paraformaldehyde 95% (Sigma, Cat#158127) Antibodies, wash buffers, etc iPS Cell Medium—DMEM/F12, 20% Knockout SR, 10 ng/ml FGFb, 1× non-essential amino acids, 1× Pen/Strep, 0.1 mM beta-Mercaptoethanol (bME), 1× GLUTAMAXTM mTeSR 1 Medium—mTeSR 1 plus 1× Supplement Materials and Methods for Characterizing iPSC Colonies Generated Using the Methods.

    Transfection:

    Article Title: Making and using in vitro-synthesized ssRNA for introducing into mammalian cells to induce a biological or biochemical effect
    Article Snippet: StainAlive DyLight 488 Mouse anti-Human Tra-1-60 Antibody (Stemgent, Cat#09-0068) Y27632 Rock I Inhibitor (Stemgent, Cat#04-0012) 10×PBS without calcium or magnesium (Lonza Biowhittaker, Cat#17-517Q, Thermo) Collagenase Type IV, 250 U/mg (GIBCO, Cat#17104-019) iMEF-irradiated mouse embryonic fibroblasts (R&D Systems Cat# PSC001) BD MATRIGELTM hESC-qualified Matrix (BD Cat#354277, Thermo) mTeSR® 1 Medium Kit—Basal Medium plus 5× Supplement (STEMCELL Technologies, Cat#05850) Dispase 5 mg/ml (STEMCELL Technologies, Cat#07913) Synth-a-Freeze, cell freezing media, (GIBCO, Cat# A12542-01, Life Technologies) DMEM/F12 (1:1) Media (GIBCO, Cat#11330, Life Technologies) KNOCKOUTTM SR Serum Replacement for ES cells (GIBCO, Cat#10828, Life Technologies) MEM Non-Essential Amino Acids Solution NEAA (100×) (GIBCO, Cat#11140, Life Technologies) Beta-mercaptoethanol (Sigma, Cat#63689) Alkaline Phosphatase Staining Kit II (Stemgent, Cat#00-0055) Bovine Serum Albumin (for FGFb) Paraformaldehyde 95% (Sigma, Cat#158127) Antibodies, wash buffers, etc iPS Cell Medium—DMEM/F12, 20% Knockout SR, 10 ng/ml FGFb, 1× non-essential amino acids, 1× Pen/Strep, 0.1 mM beta-Mercaptoethanol (bME), 1× GLUTAMAXTM mTeSR 1 Medium—mTeSR 1 plus 1× Supplement Materials and Methods for Characterizing iPSC Colonies Generated Using the Methods.

    Recombinant:

    Article Title: Making and using in vitro-synthesized ssRNA for introducing into mammalian cells to induce a biological or biochemical effect
    Article Snippet: StainAlive DyLight 488 Mouse anti-Human Tra-1-60 Antibody (Stemgent, Cat#09-0068) Y27632 Rock I Inhibitor (Stemgent, Cat#04-0012) 10×PBS without calcium or magnesium (Lonza Biowhittaker, Cat#17-517Q, Thermo) Collagenase Type IV, 250 U/mg (GIBCO, Cat#17104-019) iMEF-irradiated mouse embryonic fibroblasts (R&D Systems Cat# PSC001) BD MATRIGELTM hESC-qualified Matrix (BD Cat#354277, Thermo) mTeSR® 1 Medium Kit—Basal Medium plus 5× Supplement (STEMCELL Technologies, Cat#05850) Dispase 5 mg/ml (STEMCELL Technologies, Cat#07913) Synth-a-Freeze, cell freezing media, (GIBCO, Cat# A12542-01, Life Technologies) DMEM/F12 (1:1) Media (GIBCO, Cat#11330, Life Technologies) KNOCKOUTTM SR Serum Replacement for ES cells (GIBCO, Cat#10828, Life Technologies) MEM Non-Essential Amino Acids Solution NEAA (100×) (GIBCO, Cat#11140, Life Technologies) Beta-mercaptoethanol (Sigma, Cat#63689) Alkaline Phosphatase Staining Kit II (Stemgent, Cat#00-0055) Bovine Serum Albumin (for FGFb) Paraformaldehyde 95% (Sigma, Cat#158127) Antibodies, wash buffers, etc iPS Cell Medium—DMEM/F12, 20% Knockout SR, 10 ng/ml FGFb, 1× non-essential amino acids, 1× Pen/Strep, 0.1 mM beta-Mercaptoethanol (bME), 1× GLUTAMAXTM mTeSR 1 Medium—mTeSR 1 plus 1× Supplement Materials and Methods for Characterizing iPSC Colonies Generated Using the Methods.

    Concentration Assay:

    Article Title: Making and using in vitro-synthesized ssRNA for introducing into mammalian cells to induce a biological or biochemical effect
    Article Snippet: StainAlive DyLight 488 Mouse anti-Human Tra-1-60 Antibody (Stemgent, Cat#09-0068) Y27632 Rock I Inhibitor (Stemgent, Cat#04-0012) 10×PBS without calcium or magnesium (Lonza Biowhittaker, Cat#17-517Q, Thermo) Collagenase Type IV, 250 U/mg (GIBCO, Cat#17104-019) iMEF-irradiated mouse embryonic fibroblasts (R&D Systems Cat# PSC001) BD MATRIGELTM hESC-qualified Matrix (BD Cat#354277, Thermo) mTeSR® 1 Medium Kit—Basal Medium plus 5× Supplement (STEMCELL Technologies, Cat#05850) Dispase 5 mg/ml (STEMCELL Technologies, Cat#07913) Synth-a-Freeze, cell freezing media, (GIBCO, Cat# A12542-01, Life Technologies) DMEM/F12 (1:1) Media (GIBCO, Cat#11330, Life Technologies) KNOCKOUTTM SR Serum Replacement for ES cells (GIBCO, Cat#10828, Life Technologies) MEM Non-Essential Amino Acids Solution NEAA (100×) (GIBCO, Cat#11140, Life Technologies) Beta-mercaptoethanol (Sigma, Cat#63689) Alkaline Phosphatase Staining Kit II (Stemgent, Cat#00-0055) Bovine Serum Albumin (for FGFb) Paraformaldehyde 95% (Sigma, Cat#158127) Antibodies, wash buffers, etc iPS Cell Medium—DMEM/F12, 20% Knockout SR, 10 ng/ml FGFb, 1× non-essential amino acids, 1× Pen/Strep, 0.1 mM beta-Mercaptoethanol (bME), 1× GLUTAMAXTM mTeSR 1 Medium—mTeSR 1 plus 1× Supplement Materials and Methods for Characterizing iPSC Colonies Generated Using the Methods.



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